FIG. 2.

Vpu binding to CD74 cytoplasmic domain peptide. (A) In vitro-synthesized ³⁵S-labeled Vpu proteins retained in wells coated with either a CD74 cytoplasmic domain peptide or a nonspecific peptide (ACTH). The results shown are averages of triplicate measurements. Autoradiograms on the right show crude in vitro translation mixtures run together with molecular size markers. (B) Purified MBP-Vpu or MBP control retained in wells coated with either a CD74 cytoplasmic domain peptide or the nonspecific ACTH peptide. The results shown are averages of triplicate measurements. The Coomassie blue-stained SDS gel on the right shows purified proteins.