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. 2007 Oct 24;82(2):893–902. doi: 10.1128/JVI.01373-07

FIG. 4.

FIG. 4.

Vpu alters MHC II expression in virus-infected APCs. Activated U937 cells were infected with either HIV-1 NL4-3 or NL4-3U35. (A) At 24 h or 44 h postinfection, cells were fixed and labeled with anti-p24 and the L243 antibody (for mature MHC II), either without permeabilization for surface levels or after permeabilization for total levels. (B) The cells were similarly stained with anti-p24 and anti-CD74 (for immature MHC II) antibodies. Labeled cells were quantitated by flow cytometry. Single-parameter plots are shown for cells gated on p24 expression. The inset data show mean fluorescence intensities (± standard deviations) calculated over two separate experiments, each with duplicate samples.