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. 2007 Nov 7;82(2):1053–1058. doi: 10.1128/JVI.01813-07

FIG. 3.

FIG. 3.

HPV infectivity assay and L1 expression. (A) Infectious titer of the HPV31b control raft tissues and tissues treated with BaP in the presence of C8:0 (left panel) and in the absence of C8:0 (right panel). Shown is a 2% agarose gel of nested reverse transcription (RT)-PCR-amplified HPV31 E1̂E4 spliced transcript and β-actin in reactions with increasing dilutions of the virus stocks shown on the left. (B) The effect of BaP on L1 capsid protein expression as determined from Western blots. (C) Neutralization of HPV31b virus particles from BaP-treated and control raft cultures, using monoclonal H31.Ab, followed by detection of the E1̂E4 spliced transcript in the infectivity assay is shown. The H31.Ab was used at a dilution of 1:20 in HaCat medium. Virus stocks were used at the dilutions shown on the left. (D) Infectious titers of HPV16 and HPV18 control raft tissues and tissues treated with BaP. HPV16- and HPV18-positive cell lines HPV16(114/b)wt:3 and HPV18wt:4, respectively, were used to generate raft cultures for these assays. Shown are 2% agarose gels of nested RT-PCR-amplified HPV16 and HPV18 E1̂E4 spliced transcript and β-actin bands. Reactions were performed with increasing dilutions of the virus stocks shown on the left of each panel.