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Complementation of a gD-null virus by gD mutants. L cells were transfected to express WT gD or mutants. Transfected cells were then infected with the gD-null virus KOS-gDβ. Cells were freeze-thawed to release intracellular virus, and the virion-containing lysates were used to infect the gD-complementing cell line VD60. The results represent the averages of three independent experiments, with their standard errors shown as error bars. V, vector-transfected cells.
