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. 2007 Nov 7;82(2):692–699. doi: 10.1128/JVI.01155-07

FIG. 7.

FIG. 7.

Interaction of SHDAg-NoLS mutant and cellular RNA Pol II. (A) Huh7 cells transfected with expression plasmids for SHDAg (clone d) or clone d-2 were harvested at 48 h posttransfection. Cells were lysed with radioimmunoprecipitation assay buffer, and the lysates were immunoprecipitated with anti-HDAg antibodies. The precipitants were analyzed. (B) The same experiment as shown in panel A except that the transfected cells were treated with 0.1 μg/ml actinomycin D (ActD) at 42 h posttransfection. P, positive control; H7, untransfected Huh7 cells; WB, Western blotting; IP, immunoprecipitation; α, anti.