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. 2007 Oct 24;82(2):817–827. doi: 10.1128/JVI.01847-07

FIG. 2.

FIG. 2.

HAstV-1 virions suppress complement activity in a hemolytic complement assay, with similar kinetics to those of CVF. (A) Antibody-sensitized sheep RBCs were incubated with 20 μl NHS alone or in the presence of 1 μg CVF (black bars), along with increasing amounts of infected CaCo-2 cell lysates containing HAstV-1 virions (shaded bars) or uninfected CaCo-2 cell lysates (white bars). Hemolysis was standardized to 100% for NHS alone. One microliter of infected cell culture lysate corresponds to 3.44 × 106 genome copies, as determined by real-time RT-PCR. (B) CaCo-2 cell lysates containing HAstV-1 virions (85 μl of cell lysate, corresponding to 2.92 × 108 genome copies) and 1 μg CVF were incubated for 0 to 60 min in the presence of 20 μl NHS. At 0, 5, 15, 30, and 60 min, aliquots were removed and incubated with sensitized sheep RBCs to test hemolytic complement activity. Data are the means from five independent experiments. Error bars denote standard errors of the means (SEM).