FIG. 3.

SDS-PAGE and immunoblot analysis of fractions obtained during the sucrose gradient ultracentrifugation step of HAstV-1 CP purification. The first 18 fractions of the gradient were run in SDS-PAGE gels and stained with Coomassie blue (A) or subjected to immunoblotting with antibody to HAstV-1 virions (B). Numbers refer to gradient fractions, with arrows representing the direction of sedimentation. Molecular mass markers (in kDa) are indicated in the middle, while the position of CP is indicated to the right.