FIG. 5.

HAstV-1 CP and virions strongly suppress classical pathway activity. (A) Antibody-sensitized sheep RBCs were incubated with NHS (white bars) or fBD serum (shaded bars) in the presence of the indicated amounts of CP. (B) Antibody-sensitized sheep RBCs were incubated with fBD serum alone or in the presence of HAstV-1 virions (85 μl of cell lysate, corresponding to 2.92 × 10⁸ genome copies). Hemolysis was standardized to 100% for each serum in the absence of CP or virus. Data are the means for four (A) or three (B) independent experiments. Error bars denote SEM.