TABLE 1.
Mapping of the E1 epitope by ELISA
| MAba | Optical densityb with peptidec:
|
||||
|---|---|---|---|---|---|
| 313-332 | 288-327 | 307-340 | 317-322 | 320-326 | |
| IGH450 | 0.086 | 0.072 | 0.508 | 0.046 | 1.194 |
| IGH505 | 1.451 | 1.548 | 1.486 | 0.054 | 0.053 |
| IGH526 | 1.442 | 1.461 | 1.441 | 0.050 | 0.043 |
| IGH207 | 1.257 | 1.410 | 1.070 | 0.053 | 0.043 |
| IGH209 | 1.895 | 1.924 | 1.792 | 1.436 | 1.672 |
| IGH210 | 0.051 | 0.046 | 0.217 | 0.05 | 0.150 |
a
MAb IGH450 is from a human immunized with the E1 glycoprotein; IGH505 and IGH526 are from an HCV-infected human; and IGH207, IGH209, and IGH210 are from mice immunized with the E1 glycoprotein.
b
Positive results (>2 × background) are boldfaced.
c
Amino acids from the HCV genotype 1b strain BE-11.