FIG. 1.

TCR Vβ repertoires of epitope-specific CD8⁺ T lymphocytes generated by vaccination are as diverse as those induced in response to pathogenic-SHIV infection. PBMC were isolated from monkeys vaccinated with a plasmid DNA prime/rMVA boost regimen (135-97, 128-97, 95-98, and 90-98) and from monkeys infected with SHIV-89.6P (134, 144, 146, and 153). These cells were stimulated in vitro with p11C or p41A peptide and then stained and sorted with p11C or p41A tetramer, respectively. cDNAs synthesized from the RNAs extracted from these tetramer-binding CD8⁺ T-lymphocyte populations were used to determine Vβ repertoires. The Vβ repertoires of p11C (A) and p41A (B) tetramer-sorted CD8⁺ T lymphocytes from vaccinated monkeys and p11C (C) and p41A (D) tetramer-sorted CD8⁺ T lymphocytes from infected monkeys are shown.