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TCR Jβ family usage is identical in p11C and p41A epitope-specific CD8+ T lymphocytes following rMVA and rAd vaccinations. Second-round PCRs used for spectratyping analysis of epitope-specific CD8+ T-lymphocyte CDR3 regions from monkeys 90-98 and 128-97 served as templates for a third-round PCR to provide material for cloning and sequencing. CDR3 sequencing was done using the Cβ reverse primer.
