FIG. 7.

TCR Vβ repertoires of p11C and p41A epitope-specific CD8⁺ T lymphocytes focus transiently following infection with a pathogenic SHIV. PBMC from monkeys (Mm) 95-98 (A), 132-97 (B), and 135-97 (C) were isolated 4 (wk4 pi) and 19 (wk19 pi) weeks following SHIV-89.6P infection. The lymphocytes were cultured in vitro with p11C, p41A, and p68A peptides; stained with tetramers; and sorted. cDNA synthesized from the RNA extracted from these tetramer-binding CD8⁺ T-lymphocyte populations was used to evaluate Vβ repertoires.