TABLE 1.
Real-time PCR assay and spectratyping primers
| Primera | 5′-3′ sequence |
|---|---|
| VB1Q(2) | GAGAGAGCAAAAGGAAACATTCTTG |
| VB2Q | CTCCACTCTGACAGTGACCAATG |
| VB3Q | AGGAGCGCTTCTCCCTGATT |
| VB4Q | CGCCCAAACCTAACATTCTCA |
| VB5(A)Q | TCTCAGGGCGCCAGTTCT |
| VB5(B.1)Q | CTGAATGTGAGCGCCTTGTTG |
| VB5(B.1var)Q | GCTGAATGTGAGTGCCTTGTTG |
| VB5(B.2)Q | CTATAGCTCTGAGCTGAATGTGAA |
| VB5(B.3)Q | GCCAGTTCCGTGACTATCATCA |
| VB6(A)Q | CCAGAGTTTCTGACTTACTTCAATTATC |
| VB6(B)Q | ACTTACTTCAGTTATGAAGCTCAACA |
| VB6(C)Q | CCTTTATTGGTACCGACGGACT |
| VB6(D)Q | TTACTTCCAGAATGATGCTCAACG |
| VB6(E)Q | GTGTGATCCAATTTCAGGTCATACC |
| VB6(F)Q | ATTTACTTCCAAGGCACGGGTA |
| VB7(A)Q | GTTAAGAAGCCGCCGGAGA |
| VB7(B)Q | AAGAACGGGCTGAAAACAACA |
| VB7(C)Q | CCGCCGGAGCTCATGTTTG |
| VB8Q(2) | AACAAGTCTCCGATAGATGATTCAG |
| VB9(A)Q | AGTTCCATATCGCTTCTCACCTAAG |
| VB9(B)Q(2) | GCTCATTTAAATCTTCACATCAAGTCTG |
| VB9(C)Q | TTTCAAATCGCTTCTCACCTGAC |
| VB10Q(2) | TGGAGATCCAGTCCACAGAGT |
| VB11Q | GAGATTTTTCCTCTGAGTCAACAGTCT |
| VB12.1Q | TTACTCATATGGTGTTGAAGACACTGA |
| VB12.2Q | CATTACTCATATGGTGTTCCAGACACTA |
| VB12.3Q | GAGAAGTCCCCGATGGCTATG |
| VB13(A)Q | CCCCGATGGCTACAATGTCA |
| VB13(C.1)Q | GTCCCCAATGGCTACAATGC |
| VB13(C.2)Q | ACAGTGTCTCCAGATTAAACAAAAC |
| VB13(C.3)Q | ATTCATTACTCAGCTACCGAGGA |
| VB14Q | CGTCTCTCGAAAAGAGAAGAGGAAT |
| VB15Q | ATACAGTGTCTCTCGACAGGAACAG |
| VB16Q | GAGTCCGGTATGCCCAACA |
| VB17Q | AATCCTTTCCTCTCACTGTGACATC |
| VB18Q(2) | GTTTTCTGCTGAATTTCCCAAAGA |
| VB19Q | CAAGAAACGGAGCTGCACAA |
| VB20Q | CCAGGACAGGCGGTTCAT |
| VB21.3Q | CTGCAGAGAGGCTCAAAGGAGTA |
| VB22(A.1)Q | TGAAATATTTGAAGATCGATTCTCAGTC |
| VB22(A.2)Q | CTCAGACAAGTCTGAAATGTTCGAT |
| VB22(B.1)Q | AGTTTCTGGTTTACTTCTATAATGGTGA |
| VB22(B.2)Q | CCTTCTATAATGGTAAGATCTCAGAGC |
| VB23Q | TCAGTGACTATCATTCTGAACTGAACA |
| VB24Q | TCCAGGAGGCCAAACACTTCT |
| VB25Q(2) | CCCCCCAAATTCACCCTGTAG |
| C Beta QProbe | TGTTCCCACCCAAGGTCGCTGTG |
| C Beta/R | GATCTCTGCTTCTGATGGCTCAA |
a
Primers specific for rhesus monkey TCRβ chain variable and constant regions.