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Transfection of both reporter constructs supported efficient RNA replication. C33J-Y835C-UBI, C33J-Y835C-FMDV2A, and C33J-GND RNAs were transfected into FT3-7 cells. hRLuc activities were measured 4 h and 72 h posttransfection. Results are expressed as mean relative light units (RLU ± standard deviations) for three wells of a 96-well plate. The ratio of the RLU at 72 h to the RLU at 4 h is considered as the relative change in RNA replication.
