FIG. 6.
CDV and (S)-HPMPA are faithfully incorporated into DNA by vaccinia DNA polymerase. The reaction mixtures contained 32P-end-labeled primer P1 annealed to different template strands and were incubated with the indicated nucleotides (each at 10 μM) and 2.5 ng of vaccinia DNA polymerase/μl at 37°C (dCTP and CDVpp) or 25°C [dATP and (S)-HPMPApp]. The nucleotide encoded by the template strand at the position immediately after the primer terminus is indicated on the figure. Each reaction was stopped after 1 min, and the products were separated by gel electrophoresis and detected by using a phosphorimager.