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. 2007 Dec 10;52(2):402–408. doi: 10.1128/AAC.01005-07

FIG. 4.

FIG. 4.

Surface plasmon resonance binding titrations depicting the interactions of azurin and Laz with GST-SAG1. (A) Schematic diagram displaying the azurin proteins used in the SPR assay. Note that Laz has an additional 39 amino acids, termed the H.8 epitope, in the N-terminal region of the Neisserial azurin protein. (B) SPR time traces for the injection of 100 nM of the azurin proteins (50 μl) onto a GST-SAG1-CM5 sensor chip at a flow rate of 30 μl/min after subtraction of nonspecific binding to the negative channel (i.e., bare Au CM6 chip). (C) Concentration-dependent binding of the azurin proteins to GST-SAG1 was determined via injection of various amounts (2.7 to 150 nM) over the sensor surface, and the extent of binding was evaluated as a function of the equilibrium resonance response value measured in resonance units. The data table lists the Kd value (nM) for binding of the analyte sample to GST-SAG1.