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Egress of GS-9148-DP following the incubation of activated CD4+ lymphocytes with 0.3 μM GS-9131 for 24 h. Following the removal of GS-9131 from culture medium, GS-9148-DP persisted in activated CD4+ lymphocytes, with a half-life of 19 h. Results represent the means ± standard deviations of two independent experiments done in duplicate, with cells pooled from four donors. Data were fitted to a single exponential decay curve.
