FIG. 5.
Western blot analysis of mitochondrial proteins. Cells were exposed to the 100-fold the Cmax values of ETV or LVD or 10-fold the Cmax of ddC for 10 days. Cells were processed and analyzed as described in Materials and Methods. (A) Western blot detection of mitochondrially encoded COX II or nuclear encoded COX IV and the mitochondrial porin protein. (B) The levels of COX II and COX IV proteins were normalized according to the levels of porin protein and plotted with respect to the levels in DMSO vehicle-treated cells. A representative Western blot is shown, and the average results of three independent experiments, performed in duplicate, are plotted as means ± the SEM.