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Effect of growth temperature on the phenotypic expression of methicillin resistance (A) and growth rate (B) in strain COL. For the determination of methicillin resistance, cultures grown overnight in TSB were plated at different cell concentrations on agar plates containing twofold concentrations of methicillin. Colonies were counted after 48 h of incubation at 37 and 42°C. Growth rates were measured in TSB cultures, which were monitored by determining the A620.
