TABLE 3.
Organization and analysis of lactonamycin gene cluster from S. rishiriensis
| Gene designation | Size (aa)a | Protein homolog(s) [GenBank accession number(s)], % similarity/% identity | Possible function(s) |
|---|---|---|---|
| orf1 | 288 | SCC61A.21c (CAB892266), 88/79 | Tyrosinase |
| orf2 | 98 | SteO22.21c (CAI59990), 86/78 | Putative small membrane protein |
| orf3 | 92 | Dace_2175 (EAT15876), 58/32 | Acyl carrier protein |
| orf4 | 360 | RHA1_ro02212 (ABG94019), 51/38 | Phosphatase, acyltransferase |
| orf5 | 191 | EAV71565 (CcelDRAFT_0530), 56/35 | N-Acetyltransferase |
| orf6 | 409 | RPD_3346 (ABE40570), 55/41 | Hypothetical protein, methyltransferase? |
| orf7 | 126 | CYA_0574 (ABC98790), 61/47 | S-Adenosylmethionine decarboxylase |
| lct8 | 298 | AAP09275 (BC_2311) 48/34 | 4′-Phosphopantetheinyltransferase |
| lct9 | 314 | Orf85 (BAC76543), 82/78 | AfsA homolog, β-ketoacyltransferase, γ-butyrolactone biosynthesis |
| lct10 | 442 | Orf84 (BAC76542), 75/62 | Cytochrome P450, γ-butyrolactone biosynthesis? |
| lct11 | 254 | Orf83 (BAC76541), 75/66 | Phosphatase, γ-butyrolactone biosynthesis |
| lct12 | 249 | SngB (AAX97701), 72/61 | Ketoacyl acyl carrier protein/CoA reductase, γ-butyrolactone biosynthesis |
| lct13 | 222 | TarA (AAF06961), 82/72; TylP (AAD40801), 78/68 | γ-Butyrolactone binding protein |
| lct14 | 160 | TylQ (AAD40803), 63/50 | Transcriptional regulator |
| lct15 | 276 | Orf75 (NP_851497), 79/69; TylS (AAD40804), 75/65 | Streptomyces antibiotic regulatory protein (SARP) |
| lct16 | 500 | SAV3380 (NP_824557), 64/52 | FAD-dependent monooxygenase |
| lct17 | 327 | Orf21 (BAC79034), 73/66 | Carbohydrate kinase (glucose kinase) |
| lct18 | 1,133 | Orf49 (BAC76507), 92/87 | 5-Methyltetrahydrofolate:homocysteine S-methyltransferase |
| lct19 | 285 | Orf (+9) (AAN85549), 87/81 | 5,10-Methylenetetrahydrofolate reductase |
| lct20 | 469 | MtmH (CAK50773), 97/93 | Adenosylhomocysteinase |
| lct21 | 220 | Orf71 (BAC76569), 64/53 | SARP |
| lct22 | 509 | SAV3632 (BAC71344), 53/42 | AfsR-like regulatory protein |
| lct23 | 563 | SnorA (CAA12016), 51/38 | AfsR-like regulatory protein |
| lct24 | 92 | Sim4 (AAL15582), 69/52 | Acyl carrier protein |
| lct25 | 349 | TcmN (P16559), 66/50; FdmN (AAQ08925), 58/40 | O-Methyltransferase; aligns with the C-terminal region of TcmN |
| lct26 | 83 | RPC_2301 (ABD87855), 67/47 | Acyl carrier protein |
| lct27 | 152 | FdmI (AAQ08919), 73/59; TcmN (P16559), 68/53 | Polyketide cyclase; aligns with the N-terminal region of TcmN |
| lct28 | 510 | SAV1250 (BAC68960), 63/52 | Carboxylic acid adenylation |
| lct29 | 109 | ElmI (AAF73052), 63/42; TcmI (P39890), 63/43 | Polyketide cyclase |
| lct30 | 138 | TcmJ (P16558), 70/49; ElmJ (AAF73053), 65/48 | Polyketide cyclase |
| lct31 | 421 | TcmK (P16538), 80/69 | KSα |
| lct32 | 406 | TcmL (P16539), 74/64 | KSβ |
| lct33 | 115 | TcmH (P39889), 66/52; ElmH (AAF73051), 64/42 | Monooxygenase |
| lct34 | 248 | G111934 (BAC89875), 51/35 | Thioesterase |
| lct35 | 322 | TcmO (P39896), 61/50 | O-Methyltransferase |
| lct36 | 429 | UrdGT1a (AAF00214), 66/51 | Glycosyltransferase |
| lct37 | 139 | G113466 (BAC91407), 62/40 | Hypothetical protein, ester cyclase |
| lct38 | 493 | SAV1847 (BAC69558), 68/49 | Membrane efflux protein |
| lct39 | 332 | SCO6554 (CAA22358), 61/45 | Transcriptional regulatory protein |
| lct40 | 252 | SCO7810 (CAC03628), 70/55 | Oxidoreductase |
| lct41 | 558 | TcmG (P39888), 63/46; ElmG (AAF73050), 61/46 | Dioxygenase |
| lct42 | 108 | ElmH (AAF73051), 60/38; TcmH (P39889), 57/44 | Monooxygenase |
| lct43 | 308 | SAV4759 (BAC72471), 55/43 | Oxidoreductase |
| lct44 | 337 | LanT (ADD13550), 74/63 | NDP-hexose 3-ketoreductase |
| lct45 | 468 | LanS (AAD13549), 76/70; UrdS (AAF72552), 76/68 | NDP-hexose 2,3-dehydratase |
| lct46 | 427 | UrdQ (AAF72550), 91/80; LanQ (AAD13547), 90/80 | NDP-hexose 3-dehydratase |
| lct47 | 310 | LanZ3 (AAD13561), 56/44; UrdZ3 (AFF72549), 53/44 | NDP-hexose 4-ketoreductase |
| lct48 | 326 | LanH (AAD13546), 84/73; UrdH (AAF00211), 80/67 | NDP-hexose 4,6-dehydratase |
| lct49 | 355 | LanG (AAD13545), 77/65; UrdG (AAF00210), 76/63 | NDP-hexose synthetase |
| lct50 | 161 | LanZ1 (AAD13558), 80/69, UrdZ1 (AAF00208), 71/59 | NDP-hexose 3,5-epimerase |
| lct51 | 158 | Glr0227 (BAC88168), 55/38 | Hypothetical protein, ester cyclase |
| lct52 | 405 | MetK (AAD22464), 91/84 | S-Adenosylmethionine synthetase |
| lct53 | 542 | SCO6284 (CAC37885), 88/80 | Probable acyl-CoA decarboxylase |
| lct54 | 194 | SAV5143 (BAC72855), 69/49 | ArsR regulatory protein |
| lct55 | 378 | SCO3305 (CAB45339), 69/52 | Integral membrane acyltransferase |
| lct56 | 297 | SAV4467 (BAC72179), 90/85 | rRNA methyltransferase, lactonamycin resistance? |
| orf57 | 322 | SAV4466 (BAC72178), 91/82 | Putative IS1650 family transposase |
a
aa, amino acids.