FIG. 3.

Relative expression levels of TNFA, IL-1B, and DECTIN-1 mRNAs after 1 h of exposure of HPMNs to hyphae of Af293 or R. oryzae, as assessed by RT-PCR. Forward and reverse PCR primers for human TNF-α (5′-AGG CCA AGC CCT GGT ATG AGC-3′ and 5′-CAC AGG GCA ATG ATC CCA AAG TAG-3′), IL-1β (5′-CAG GGA CAG GAT ATG GAG CAA CAA-3′ and 5′-CAT CTT TCA ACA CGC AGG ACA GGT-3′), Dectin-1 (5′-TGG CAA CTG GGC TCT AAT CTC CT-3′ and 5′-TTT CTT GGG TAG CTG TGG TTC TGA-3′) and beta-actin (5) were used to simultaneously amplify cDNA and the housekeeping gene using multiplex chemistry. The products were subjected to 32 cycles of PCR amplification at 94°C for 30 s, 58°C for 30 s, and 72°C for 2 min. P was nonsignificant for all comparisons. The error bars indicate standard deviations.