FIG. 1.

Inhibitory activities of moxifloxacin (A), garenoxacin (B), and novobiocin (C) on the supercoiling activities of M. leprae wild-type (WT) and mutant DNA gyrases. Relaxed pBR322 (0.4 μg) was incubated with DNA gyrases reconstituted from wild-type or mutant subunits (carrying mutations G89C and A91V in GyrA and D205N in GyrB) in the absence or presence of the indicated amounts (in μg/ml) of moxifloxacin (MOX), garenoxacin (GAR), or novobiocin (NOV). Reactions were stopped, and the DNA products were analyzed by electrophoresis in 1% agarose gel. Lane TR represents relaxed pBR322 DNA. R and S denote relaxed and supercoiled DNAs, respectively.