FIG. 2.

Quinolone (moxifloxacin)-mediated DNA cleavage by M. leprae wild-type (WT) and mutant DNA gyrases. Supercoiled pBR322 DNA (0.4 μg) was incubated with DNA gyrases reconstituted from wild-type or mutant subunits (carrying mutation A91V in GyrA or D205N in GyrB) in the absence or presence of the indicated amounts (in μg/ml) of moxifloxacin (MOX). After addition of sodium dodecyl sulfate and proteinase K, DNA samples were analyzed by electrophoresis in 1% agarose. Lane TS represents supercoiled pBR322 DNA. R, N, and S denote relaxed, nicked, and supercoiled DNAs, respectively.