Mass spectra of subtilin, N-succinylated subtilin, and subtilin fragments. (A) Subtilin, m/z 3,321 Da; (B) N-succinylated subtilin, m/z 3,421 Da; (C) subtilin fragment 1-29, m/z 3,010 Da; (D) subtilin fragment 2-29, m/z 2,824 Da. The observed molecular ion at m/z 2,466 Da is due to an internal reference, human adrenocorticotropic hormone fragment 18-39.