TABLE 1.
Primers used in this study
| Primer | Sequence (5′→3′) | Location in C. difficile CD5 tet(W) regiona |
|---|---|---|
| Detection and sequencing of tet(W) gene | ||
| WRC1 | CATCTCTGTGATTTTCAGCTTTTCTCTCCC | 1871-1897 |
| WRC2 | AGTCTGTTCGGGATAAGCTCTCCGCCG | 2328-2299 |
| W9 | ATGAAAATAATCAATATTGGAATTCT | 1447-1472 |
| W11 | TTACATTACCTTCTGAAACATATG | 3366-3343 |
| Sequencing of tet(W) flanking regions | ||
| TSP1 | GAGAGCTTATCCCGAACAGA | 1892-1873 |
| TSP2 | CATCTGTGCCACTGGAAGGA | 1649-1630 |
| TSP3 | GCTCTCCGTCAAGGTCGTCT | 1512-1493 |
| TER1 | GTGTTGTCTGCATTTTACT | 979-961 |
| AUN1 | GGTGAGCCGGATTGGG | NR |
| TSP4 | TGGAACGTAACGGACTGTAA | 2920-2939 |
| TSP5 | CTCTATGCGCCCCAGGAATA | 3076-3095 |
| TSP6 | GCGGAGCGTATGCCTTACAG | 3243-3262 |
| TSP6A | TTAAGGGCTTGTCCTCTCTGCC | 3642-3663 |
| Wrev6 | GCTCTGCGTCTATGCGTCT | 4046-4064 |
| Wrev4 | AGATGTTCCTCGCGCAATTTT | 4497-4517 |
| Wrev2 | ATGAAACGTTTACCTAAATATAC | 5059-5037 |
| UNI3A | TCACAGAAGTATGCCAAGCGA | NR |
a
NR, the positions of AUN1 and UNI3A are not reported since these primers were used to sequence the 5′ and the 3′ ends of the C. difficile CD5 tet(W) region, respectively.