TABLE 1.

Susceptibilities of C. albican strains against azoles alone and in combination with FK506 obtained by the spectrophotometric method^a

Drug in combination and strain	Median MIC (range) of drug (μg/ml)
	Alone		In combination
	Azole	FK506	Azole	FK506
FLC
Susceptible
CA5	0.25 (0.25-0.5)	512	0.25 (0.125-0.5)	0.002 (0.002)
CA8	0.25 (0.125-0.5)	512	0.125 (0.125-0.25)	0.002 (0.002)
CA12	4 (2-4)	512	2 (1-2)	0.008 (0.004-0.008)
CA14	0.5 (0.25-0.5)	512	0.25 (0.25-1)	0.002 (0.002-0.004)
CA129	0.5 (0.5-1)	512	0.25 (0.25-0.5)	0.008 (0.004-0.016)
Resistant
CA10	256 (256-512)	512	0.25 (0.125-0.5)	0.063 (0.016-0.125)
CA15	128 (64-128)	512	0.5 (0.25-0.5)	0.008 (0.004-0.016)
CA16	64 (64-128)	512	0.5 (0.25-2)	0.008 (0.008-0.016)
CA135	64 (64-128)	512	0.25 (0.125-0.5)	0.004 (0.004-0.008)
CA137	64 (64-128)	512	0.5 (0.25-0.5)	0.002 (0.002-0.008)
ITR
Susceptible
CA5	0.031 (0.031-0.063)	512	0.031 (0.031-0.063)	0.063 (0.016-0.063)
CA8	0.016 (0.008-0.031)	512	0.008 (0.008-0.016)	0.002 (0.002)
CA12	0.063 (0.063-0.125)	512	0.031 (0.031-0.063)	0.125 (0.063-0.125)
CA14	0.063 (0.031-0.125)	512	0.031 (0.031-0.125)	0.002 (0.002)
CA129	0.063 (0.063-0.125)	512	0.031 (0.031-0.063)	0.063 (0.063-0.125)
Resistant
CA10	128 (64-128)	512	0.031 (0.031-0.063)	0.125 (0.063-0.125)
CA15	32 (16-64)	512	0.063 (0.031-0.125)	0.063 (0.031-0.125)
CA16	32 (16-64)	512	0.016 (0.008-0.031)	0.125 (0.125)
CA135	16 (16-32)	512	0.016 (0.016-0.031)	0.063 (0.031-0.125)
CA137	16 (16-32)	512	0.063 (0.031-0.063)	0.008 (0.008-0.031)
VRC
Susceptible
CA5	0.016 (0.016-0.031)	512	0.016 (0.016-0.031)	0.002 (0.002)
CA8	0.008 (0.008-0.016)	512	0.016 (0.008-0.031)	0.002 (0.002)
CA12	0.031 (0.008-0.031)	512	0.016 (0.008-0.031)	0.008 (0.004-0.016)
CA14	0.008 (0.004-0.016)	512	0.008 (0.008)	0.002 (0.002)
CA129	0.016 (0.016-0.031)	512	0.008 (0.008-0.016)	0.008 (0.008-0.125)
Resistant
CA10	64 (32-64)	512	0.008 (0.008-0.016)	0.063 (0.031-0.063)
CA15	8 (4-16)	512	0.008 (0.008-0.016)	0.125 (0.063-0.125)
CA16	8 (4-8)	512	0.008 (0.008-0.016)	0.031 (0.031-0.063)
CA135	8 (4-8)	512	0.016 (0.008-0.031)	0.031 (0.016-0.063)
CA137	8 (4-16)	512	0.008 (0.008-0.016)	0.016 (0.008-0.016)

^aStrains were categorized as susceptible or resistant according to the interpretive breakpoints of CLSI criteria for FLC (≤8 and ≥64 μg/ml, respectively), ITR (≤0.1 and ≥1 μg/ml, respectively), and VRC (≤1 and ≥4 μg/ml, respectively) (11). For azole-susceptible isolates, when drugs were used in combination, MICs of azoles showed no apparent changes, while for azole-resistant strains, MICs of azoles decreased markedly when drugs were used in combination.