Table 2.
Brefeldin A prevents the Bcl-xL–DTR blockade of apoptosis
| Compound | Cell death, % | Protection, % |
|---|---|---|
| PBS (control) | 1 | |
| STS | 24 | |
| STS + Bcl-xL–DTR | 11 | |
| Bcl-xL–DTR | — | 56 |
| Brefeldin A | 2 | |
| STS + brefeldin A | 35 | |
| STS + brefeldin A + Bcl-xL–DTR | 32 | |
| Bcl-xL–DTR + brefeldin A | — | 9 |
Apoptotic cells were counted with Hoechst dye no. 33342 14 hr after addition of STS and/or brefeldin A minutes after Bcl-xL–DTR was added to Cos-7 cells. STS was used at 0.1 μM; Bcl-xL–DTR was used at 2.24 μM; brefeldin A was used at 2 μM.