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A 12% SDS–PAGE gel stained with Coomassie Brilliant Blue showing the apparent homogeneity of the purified EhMGL2. Lane 1, E. coli soluble extract before IPTG induction; lane 2, E. coli soluble extract after IPTG induction; lane 3, eluate from the GSTrap column; lane 4, purified EhMGL2 after HPLC chromatography using a MonoQ column; lane M, molecular-weight markers (kDa).
