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Effect of thiostrepton on EF-G-dependent translocation at 20°C (a) and 37°C (b). Pretranslocation complexes (0.2 μM) were incubated with 0.04 μM (●, ○) or 0.6 μM (■, □) EF-G⋅GTP in the absence (○, □) or presence (●, ■) of 100 μM thiostrepton. ▿, No EF-G added.
