Table 1.
Enzyme | ν4 | ν11(a) | ν2 (a) | νC=O (formyl, a3) |
---|---|---|---|---|
Purified, t = 1 min* | 1,355 | 1,520 | 1,587 | 1,660 |
Purified, t ∼ 30 min | 1,355 | 1,520 | 1,587 | 1,667 |
Purified + UQ, t ∼ 10 min† | 1,355 | 1,520 | 1,587 | 1,667 |
Intact membrane | 1,355 | 1,519 | 1,587 | 1,667 |
Membrane extract‡ | 1,356 | 1,519 | 1,588 | 1,667.5 |
Bovine§ | 1,354 | 1,517 | 1,586 | 1,664 |
The enzyme samples were reduced by addition of a degassed dithionite solution under anaerobic conditions.
Purified aa3in 100 mM phosphate buffer, pH 7.4, 0.3 mg/ml lauryl maltoside, spectrum recorded ∼1 min after reduction by dithionite.
The purified oxidized aa3 was preincubated with 1.6 mg/ml decylubiquinone (UQ) for 1 hr and then was reduced by dithionite. Spectrum was recorded after 10 min.
Membranes dissolved in lauryl maltoside (2g LM/1 g protein).
Data from Argade et al. (30) on bovine cytochrome c oxidase.