Table 1.

Selected resonance Raman frequencies (Ex. 413.1 nm) of various preparations of A. ambivalens aa₃ quinol oxidase in the reduced state

Enzyme	ν4	ν11(a)	ν2 (a)	νC=O (formyl, a3)
Purified, t = 1 min*	1,355	1,520	1,587	1,660
Purified, t ∼ 30 min	1,355	1,520	1,587	1,667
Purified + UQ, t ∼ 10 min†	1,355	1,520	1,587	1,667
Intact membrane	1,355	1,519	1,587	1,667
Membrane extract‡	1,356	1,519	1,588	1,667.5
Bovine§	1,354	1,517	1,586	1,664

The enzyme samples were reduced by addition of a degassed dithionite solution under anaerobic conditions. 

^*Purified aa₃in 100 mM phosphate buffer, pH 7.4, 0.3 mg/ml lauryl maltoside, spectrum recorded ∼1 min after reduction by dithionite. 

^†The purified oxidized aa₃ was preincubated with 1.6 mg/ml decylubiquinone (UQ) for 1 hr and then was reduced by dithionite. Spectrum was recorded after 10 min. 

^‡Membranes dissolved in lauryl maltoside (2g LM/1 g protein). 

^§Data from Argade et al. (30) on bovine cytochrome c oxidase.