Fig. 2.

LacZ expression of enhancer trap lines that modify leg disc regeneration. PZ insertions lines were examined for lacZ expression in the regeneration blastema (proximodorsal region) following ubiquitous wg expression. (A) Pie-chart shows the distribution of the 90 PZ insertions into four different classes of lacZ expression: Class 1 lines show no expression change upon ubiquitous wg expression (36%, blue), Class 2 lines show partial or complete loss of expression (13%, light blue), Class 3 lines exhibit ubiquitous expression (28%; yellow) and Class 4 lines display expression limited to the regeneration blastema (23%; red). (B) Pie-chart shows the lacZ expression patterns of the 19 PZ insertions identified to modify wg-induced leg-to-wing transdetermination. Note that there is an enrichment for genes that exhibit blastema-specific expression following ubiquitous wg expression (Class 4, 42%; red). (C) Representative examples of the four classes of lacZ expression patterns. Upper panels show endogenous expression in prothoracic leg disc pairs from four PZ lines [ken and barbie (ken), oho23B, Krüppel-homolog-1 (Kr-h1), and l(3)01629]. The leg discs were dissected from wandering third instar larvae. Lower panels show lacZ expression of ken, oho23B, Kr-h1, and l(3)01629 after ubiquitously expressing wg (Act > wg) during the mid-second instar. Leg disc pairs were dissected 2.5 days (120 h AED) after wg induction. Red arrowheads mark lacZ expression in the chordotonal organ. Scale bar, 50 μm.