. 2006 Nov 30;62(Pt 12):1235–1238. doi: 10.1107/S1744309106046070

Table 1. The optimized crystallization conditions for the various constructs of IPNV VP4 protease.

Crystal	Form 1	Form 2	Form 3	Form 4	Form 5
VP4 construct	514–734-Hx6	514–715,K674A	514–716,K674A	514–716,K674A	524–716,K674A
Concentration (mg ml⁻¹)	30	48	58	59	30
Temperature (K)	277	298	277	277	291
Crystallization conditions	0.1 M Tris pH 8.5, 20% PEG 2K MME, 2 M NaCl	0.1 M Na citrate pH 5.6, 1.0 M (NH₄)H₂PO₄, 3%(w/v) D-(+)-sucrose	0.1 M MES pH 6.0, 20% PEG 2K MME, 3 M NaCl	0.1 M Tris pH 8.5, 31% PEG 4K, 0.4 M Li₂SO₄, 0.2 M guanidine–HCl	0.1 M Tris pH 8.5, 17% PEG 3350, 0.4 M Ca acetate
Maturation time (weeks)	3–4	2–3	3–4	1–2	1–2
Cryoconditions	0.1 M Tris pH 8.5, 20% PEG 2K MME, 2 M NaCl, 10% glycerol	0.1 M Na citrate pH 5.6, 1.0 M (NH₄)H₂PO₄, 30% glycerol	0.1 M MES pH 6.0, 30% PEG 2K MME, 1.2 M NaCl	0.1 M Tris pH 8.5, 35% PEG 4K, 0.45 M guanidine–HCl, 10% glycerol	0.1 M Tris pH 8.5, 20% PEG 3350, 0.4 M Ca acetate, 10% glycerol
Diffraction	Anisotropic (∼3.0 Å)	Anisotropic (∼4.0 Å)	Anisotropic (∼3.5 Å)	Beyond 2.4 Å (see Table 2 ▶)	Beyond 2.4 Å (see Table 2 ▶)