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. 2008 Feb 5;6(2):e17. doi: 10.1371/journal.pbio.0060017

Figure 7. Lack of Seeding Capacity of L20 Fibrils on α-Syn.

Figure 7

The freshly prepared α-Syn solution in PBS (pH 7.4) was incubated with 5% (v/v) seed of matured L20 amyloid in 5% D-mannitol at 37 °C with vigorous stirring. α-Syn in PBS (pH 7.4) with 5% (v/v) of 5% D-mannitol was also incubated as a control. The formation of amyloid was monitored for the mixture (α-Syn+L20; red line), α-Syn in the presence (positive control; blue line) and the absence (negative control; black line) of α-Syn seed (5%) by Thio T binding. The intensity of Thio T fluorescence is shown as arbitrary units (AU) versus time.