Table 2.

ITC Binding Data for Phosphotyrosine Peptides

Peptide	KD (μM)	KB × 105 (M−1)	ΔHobs (kcal/mol)	TΔS (kcal/mol)	ΔG (kcal/mol)	Na
SOCS2/ElonginBC
EGFR pY1069	>15
EGFR pY1092	>15
EpoR pY402b	7.1	1.42 ± 0.27	−3.69 ± 0.46	3.34	−7.03	0.73
GHR pY487	11.3	0.89 ± 0.64	−1.49 ± 0.76	5.16	−6.65	1.05
GHR pY595b	1.6	6.08 ± 1.03	−3.35 ± 0.11	4.54	−7.89	1.22
gp130 pY759c	>15
JAK2 pY1007	8.1	1.23 ± 0.34	−3.62 ± 0.63	3.22	−6.84	0.89
KIT pY568	8.3	1.20 ± 0.23	−3.62 ± 0.38	3.19	−6.81	1.12
LeptinR pY1077	>15
Peptide pYINP	>15
SOCS4/ElonginBC
EGFR pY1069	11.6	0.86 ± 0.12	−13.0 ± 2.0	−6.35	−6.64	0.95
EGFR pY1092	0.5	19.0 ± 1.0	−10.8 ± 0.1	−2.36	−8.42	1.00
EpoR pY402	7.7	1.30 ± 0.16	−14.5 ± 1.3	−7.59	−6.86	0.71
GHR pY487	5.1	1.96 ± 0.11	−10.6 ± 0.2	−3.49	−7.10	1.18
GHR pY595	6.1	1.63 ± 0.18	−10.1 ± 0.5	−3.08	−6.99	0.96
gp130 pY759c	7.1	1.41 ± 0.17	−7.08 ± 0.40	−0.17	−6.91	1.12
JAK2 pY1007	2.9	3.43 ± 0.25	−11.3 ± 0.2	−3.87	−7.41	1.00
KIT pY568	2.9	3.43 ± 0.24	−7.03 ± 0.12	0.40	−7.43	1.29
LeptinR pY1077	5.5	1.82 ± 0.13	−7.69 ± 0.24	−0.63	−7.06	0.96
Peptide pYINP	1.1	9.40 ± 0.47	−12.2 ± 0.1	−4.16	−8.03	0.79

^aStoichiometry and curve fitting errors determined from a single binding site model with the Microcal Origin software.

^bBullock et al., 2006.

^cNote the human numbering differs from murine gp130 pY757. The preferred substrate peptide is shown in bold. ITC binding curves for these data are shown in Figure S1.