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Nuclear and cytoplasmic ROIs are applied to the original NF-κB rel A image files to extract rel A immunofluorescence data for each region in unstimulated and LPS stimulated macrophages (A). Bar represents 100 μm. Histograms of the frequency distribution of fluorescence intensity show increased nuclear:cytoplasmic rel A staining in LPS stimulated cells compared to unstimulated cells (B).
