Table 1.
Summary of nickel-interacting H. pylori proteins identified by peptide mass fingerprinting.
| Protein no. | Protein name | Identified peptides | % Sequence coverage | MOWSE score | MW (kDa)/pI | |
|---|---|---|---|---|---|---|
| Theor. | Obs. | |||||
| 1* | UreB | 21 | 42 | 1.595e+10 | 61.4/5.5 | 65.5/5.8 |
| 2* | 60 kDa chaperonin (HspB) | 65 | 75 | 1.199e+15 | 58.2/5.5 | 59.3/5.3 |
| 3 | 60 kDa chaperonin (HspB) | 19 | 37 | 9.875e+04 | 58.2/5.5 | 56.7/5.5 |
| 4 | Inosine--monophosphate dehydrogenase (GuaB) | 24 | 51 | 2.559e+09 | 51.8/7.7 | 56.2/8.0 |
| 5 | Fumarase | 22 | 51 | 1.097e+11 | 51.0/6.5 | 52.6/7.4 |
| 6 | Fumarase | 20 | 45 | 1.800e+10 | 51.0/6.5 | 52.6/7.0 |
| 7 | Putative aminotransferase | 26 | 55 | 2.466e+12 | 42.4/5.8 | 40.4/6.2 |
| 8 | Hypothetical protein HP0271 (fragment) | 4 | 12 | 980 | 38.6/5.0 | 21.1/4.8 |
| 9 | Cinnnamyl-alcohol dehydrogenase ELI3-2 (Cad) | 15 | 36 | 1.912e+09 | 38.6/7.0 | 40.7/7.8 |
| 10 | Cell binding factor 2 | 21 | 48 | 5.030e+06 | 34.0/9.3 | 31.9/9.8 |
| 11 | Outer membrane protein jhp1472 | 11 | 32 | 6.291e+04 | 30.2/9.1 | 26.7/9.3 |
| 12 | Hypothetical protein jhp0216 | 15 | 57 | 7.869e+05 | 29.5/9.1 | 27.4/9.4 |
| 13 | Hypothetical protein jhp0301 | 7 | 24 | 1.027e+04 | 28.6/7.8 | 27.1/8.0 |
| 14 | Hypothetical protein jhp0301 | 19 | 61 | 2.461e+05 | 28.6/7.8 | 27.1/8.1 |
| 15 | Putative neuraminyllactose-binding hemagglutinin homolog (HpaA) | 13 | 50 | 2.381e+05 | 28.3/7.9 | 27.0/7.2 |
| 16 | Urease alpha subunit (UreA) | 14 | 68 | 1.280e+07 | 26.5/8.5 | 27.2/9.0 |
| 17 | Urease alpha subunit (UreA) | 4 | 88 | 1.846e+11 | 26.5/8.5 | 27.2/8.5 |
| 18 | Putative alkyl hydroperoxide reductase (TsaA) | 12 | 54 | 1.297e+07 | 21.9/5.7 | 19.9/6.2 |
| 19 | Inorganic pyrophosphatase (PPase) | 4 | 23 | 575 | 14.7/5.0 | 20.7/4.7 |
| 20 | Non-heme iron containing ferritin (Pfr) | 2 | 23 | 369 | 19.3/5.4 | 14.4/5.2 |
| 21 | Non-heme rion containing ferretin (Pfr) | 8 | 56 | 2.190e+07 | 19.3/5.4 | 14.4/5.5 |
| 22 | Hypothetical protein HP0721 | 13 | 44 | 1.692e+04 | 17.6/8.9 | 14.4/8.5 |
| 23 | Neutrophil activating protein (NapA) | 6 | 48 | 1.570e+05 | 16.8/5.7 | 10.2/6.2 |
| 24 | Hypothetical protein jhp0118 | 4 | 32 | 1.620e+03 | 16.6/9.3 | 12.2/9.8 |
| 25 | Chaperonin groES (HspA) | 6 | 44 | 146 | 13.0/6.1 | 10.2/6.4 |
| 26 | Hepothetical protein HP0614 | 13 | 56 | 1.557e+05 | 13.0/7.0 | 7.7/8.0 |
| 27 | 4-oxalocrotonate tautomerase (DmpI) | 10 | 85 | 3.420e+05 | 7.5/6.0 | 5.5/6.4 |
*Confirmed with 2-DE images of H. pylori 26695 (http://www.mpiib-berlin.mpg.de/2D-PAGE/EBP-PAGE/index.html).