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. Author manuscript; available in PMC: 2008 Feb 3.
Published in final edited form as: J Biol Chem. 2005 Aug 3;280(41):34878–34887. doi: 10.1074/jbc.M501977200

Fig. 1.

Fig. 1

Peroxynitrite inhibits IκBα phosphorylation and degradation triggered by inflammatory stimuli in H9C2 cells. H9C2 cells were either not stimulated (NS) or stimulated with LPS (1μg/ml, A, C, E) or TNFα (20 ng/ml, B, D, F) after pretreatment with either decomposed peroxynitrite (DP) or authentic peroxynitrite (PN, 250 μM) for 20 min. Both LPS and TNFα induced IκBα phosphorylation (A, B) and degradation (C, D), which were prevented by PN but not DP. Expression levels of α-tubulin (E, F) are shown as loading controls.