Fig. 3.

Peroxynitrite inhibits NF-κB DNA binding activity triggered by inflammatory stimuli in H9C2, HMEC-1 and EAhy 926 cells. H9C2 cells were not stimulated (NS) or treated with LPS (1μg/ml, A) or TNFα (20 ng/ml, B). Both LPS and TNF triggered NF-κB DNA binding, an effect suppressed by pretreatment with peroxynitrite (PN, 250 μM, 20 min), but not decomposed PN (DP). The inhibitory effect of PN on LPS-mediated NF-κB DNA binding was concentration-dependent (C, D). PN, but not DP, also inhibited NF-κB DNA binding in HMEC-1 cells (E) and EAhy 926 cells (F) stimulated with TNFα (20 ng/ml), IL1β (1 ng/ml) or both. Bar graph shows mean±sem of at least 4 observations. * p<0.05 (ANOVA followed by Bonferroni adjustments)