Fig. 6.
(A) Representative Western blot analysis of human mesangial cell nitrosylated proteins. Equal protein loading was confirmed with β-actin antibody. Lane 1, 5.55 mM glucose; lane 2, 30 mM D-glucose; lane 3, 30 mM L-glucose; lane 4, 30 mM D-glucose + 10 μM fidarestat. (B) Total nitrosylated protein content in human mesangial cells cultured in 5.55 mM glucose (1), 30 mM D-glucose (2), 30 mM L-glucose (3), and 30 mM glucose + 10 μM fidarestat (4, mean ± SE, n = 4 per condition). Total nitrosylated protein content in cells cultured in 5.55 mM glucose is taken as 100%. *p < 0.05 vs cells cultured in 5.55 mM glucose; #p < 0.05 vs cells cultured in 30 mM D-glucose.