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. Author manuscript; available in PMC: 2008 Aug 1.
Published in final edited form as: Exp Hematol. 2007 Aug;35(8):1209–1218. doi: 10.1016/j.exphem.2007.05.003

Figure 3.

Figure 3

Reduction of βS-globin mRNA by ribozymes in K562βS cells. K562βS cells were transfected with different ribozyme constructs and total RNA extracted. The amounts of βS-globin mRNA, β-actin mRNA, and ribozyme RNA were determined by primer extension assays and normalized to β-actin mRNA. The βS-globin mRNA value obtained for K562βS cells transfected with pHC-0 (control plasmid) is set at 100%. RNA samples from cells transfected with (lanes 1-8, from left): pHC-0; pHC-zb1; pHC-zb2; pHC-zb3; pHC-zb4; pHC-zb5; pHC-zb21A; and pHC-zb61.