Table 1.
Compound identification | Li+ adduct (m/z) |
Normal eyea (mg μl−1) |
Dry eyeb (mg μl−1) |
Increase (%) |
---|---|---|---|---|
Free glycerol | 99 | 9.6 × 10−4 ± 2.3 × 10−4 | 1.5 × 10−3 ± 2.7 × 10−4 | 56 |
Glycerylisoprene acetal | 153 | 6.8 × 10−4 ± 2.7 × 10−4 | 1.7 × 10−3 ± 8.5 × 10−4 | 150 |
Glycerylisoprene acetal | 287 | 7.5 × 10−4 ± 2.9 × 10−4 | 1.8 × 10−3 ± 9.2 × 10−4 | 140 |
Monopalmitin | 337 | 7.4 × 10−4 ± 1.6 × 10−4 | 1.1 × 10−3 ± 2.0 × 10−4 | 49 |
Monostearin | 365 | 1.6 × 10−3 ± 3.4 × 10−4 | 2.5 × 10−3 ± 2.5 × 10−4 | 56 |
Glycerylisoprene acetal | 421 | 1.2 × 10−3 ± 3.7 × 10−4 | 2.9 × 10−3 ± 1.6 × 10−3 | 142 |
1,3-Dipalmitin (major) | 575 | 1.4 × 10−4 ± 3.1 × 10−5 | 2.1 × 10−4 ± 2.3 × 10−5 | 50 |
1-Stearin-3-myristin (minor) | ||||
1-Myristin-2-stearin (minor) | ||||
1-Stearin-3-palmitin (major) | 603 | 5.2 × 10−4 ± 9.5 × 10−5 | 7.3 × 10−4 ± 2.7 × 10−5 | 40 |
1,3-Distearin (major) | 631 | 5.1 × 10−4 ± 9.0 × 10−5 | 7.2 × 10−4 ± 1.3 × 10−5 | 41 |
Tristearin (major)c | 897 | 1.3 × 10−4 ± 5.5 × 10−5 | 1.8 × 10−4 ± 9.9 × 10−5 | 38 |
1-Arachidin-2-stearin-3-palmitin (minor) | ||||
Total | 7.3 × 10−3 ± 7.1 × 10−4 | 1.4 × 10−2 ± 2.1 × 10−3 | 92 |
Averages and standard errors of the mean reported for normal eye results were derived from four different sets of composite rabbit tear samples, where each composite set was analyzed separately. Tear composite volumes were 12, 12, 7, and 7 μL.
Dry eye results are the average and standard error of the mean of two different composite sets which were analyzed individually. Tear composite volumes were 6 and 7 μl.
Reported value for the triglyceride tristearin is substantially above the method's limit of detection for triglycerides (i.e. 5.8 × 10−6 mg μl−1).