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. Author manuscript; available in PMC: 2008 Feb 4.
Published in final edited form as: J Dent Res. 2006 Aug;85(8):739–744. doi: 10.1177/154405910608500810

Figure 4.

Figure 4

Regulation of adenosine receptor engagement by ecto-ADA and CD73. (A) Effect of XAC on adenosine (Ado) or AMP-induced HAS1 mRNA expression in human gingival fibroblasts. Human gingival fibroblasts (5.0 × 105 cells/well) were treated with Ado (20 μM) or AMP (20 μM) for 2.5 hrs in the presence or absence of XAC (5 μM). HAS1 mRNA expression in human gingival fibroblasts was examined by RT-PCR. The results shown are representative of 3 separate experiments. The number of PCR cycles is shown above each lane. Each band was standardized against the amount of HPRT. (B) Effects of ecto-ADA on AMP-induced HAS1 mRNA expression in human gingival fibroblasts. Human gingival fibroblasts were pre-incubated with or without Jurkat lysate for 30 min at 37°C, and treated with AMP (20 μM) in the presence and absence of dCF (5 μM) for 2.5 hrs. HAS1 mRNA expression in human gingival fibroblasts was examined by RT-PCR. The results shown are representative of 3 separate experiments. The number of PCR cycles is shown above each lane. Each band was standardized against the amount of HPRT.