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. Author manuscript; available in PMC: 2008 Oct 10.
Published in final edited form as: Vaccine. 2007 Aug 6;25(41):7247–7260. doi: 10.1016/j.vaccine.2007.07.038

Fig. 4.

Fig. 4

SDS-PAGE and Western blot analysis of purified Ctb chimeras. Panel A. Coomasie-stained SDS-PAGE of purified chimeric antigens. Lanes labeled NB-Ctb contained NB-A2 fusions complexed with Ctb; lanes labeled NB-L2-Ctb contained NB-L2-A2 fusions complexed with Ctb; lanes labeled CT contained commercially-available holo-cholera toxin as a control. Samples were separated by SDS-PAGE either without prior reduction and heating, or with reduction and heat (H). Panel B. Western blot analysis of purified chimeric antigens and CT using antisera specific for TbpB, TbpA, and CT. Lanes are labeled as indicated for panel A except that all samples were reduced and heated before separation by SDS-PAGE.