[A] Comparison of the immunogenicity of 25 μg DNA administered with EP [EP] or without EP [No EP]. Optical density at 405nm of individual mouse sera at 1:4,000 dilution after prime and one boost are shown. [B] shows Comparison of the immunogenicity of 25 μg DNA administered without EP [25μg, No EP] and 0.25μg with EP [0.25μg, EP]. Optical density at 405nm of individual mouse sera at 1:4,000 dilution after prime and one boost are shown. The antibody responses in the two groups, i.e., 25 μg without EP and 0.25 μg without EP were similar with a significance of 91.6% as assessed by Levenne’s test for equality of variance.