Figure 1.

Recombinant basonuclin recovered from inclusion bodies. E. coli expressing human basonuclin from plasmid pHUB2 were disrupted, and the lysate was divided into soluble and particulate fractions (S and P). Each fraction was subjected to SDS/PAGE, and the resolved proteins were stained with Coomassie blue. Human basonuclin, indicated by an arrowhead at 140 kDa (K), was the most abundant protein. This protein was absent from the particulate fraction of cells harboring the vector plasmid pET28a(+). Basonuclin was absent from both soluble fractions, which otherwise contained virtually identical proteins. M, standard markers.