Figure 2.

A human genomic fragment with affinity for basonuclin. A genomic library was subjected to selection based on affinity binding to basonuclin inclusion bodies. After each cycle of binding and elution, the DNA was amplified, digested with KpnI, and analyzed by agarose electrophoresis and ethidium bromide staining. The number above each lane indicates the selection cycle. After the fifth cycle, a single band of 5 kilobases (kb) became dominant (pTRG1). The band of 3 kb, corresponding to the size of the vector, could no longer be detected after the sixth cycle. In lane M, a λDNA–BstEII digest is shown as standard marker.