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. 1999 Aug 17;96(17):9628–9632. doi: 10.1073/pnas.96.17.9628

Figure 4.

Figure 4

Resistance to DNase I of rRNA promoter region bound to basonuclin inclusion bodies (BNI). (A) The noncoding strand (24 fmol) of the 258-bp DNA of the human rRNA promoter, to which a short stretch of vector sequence was added to label its 3′ end, was incubated with (+, 5 μg; ++, 10 μg; +++, 20 μg) or without (−) BNI and then digested with 10 milliunits of DNase I. BNI protected the BBS1 region and, less strongly, BBS2 (shown by boxes). The G + A and T + C sequence reactions were performed as described (11). (B) The coding strand (43 fmol) of the EcoRI–NheI fragment of the human rRNA promoter, labeled at the 3′ end, was incubated with (+++, 20 μg) or without (−) BNI and then digested with 12.5 milliunits of DNase I. Both BBS1 and BBS2 were protected by BNI. BBS2 was more strongly protected in this strand than in the noncoding strand.