Table 1.
Markers used for linkage analysis
| Genea | Type of markerb | Position of markerc | Alleles observed (allele frequency)d | Haplotypes observed (haplotype frequency)d |
|---|---|---|---|---|
| htr1A | (CA)n (UU160O12) |
*7370 | 297 (0.5) 303 (0.5) |
– |
| htr1B | A/C SNPe | 157 | A (0.58) C (0.42) |
143-A-G-T-G (0.20) 143-C-A-T-G (0.42) 143-A-G-C-C (0.06) 139-A-G-C-C (0.24) 139-A-G-T-G (0.05) 147-A-G-T-G (0.03) |
| G/A SNP | 246 | G (0.58) A (0.42) |
||
| T/C SNP | 955 | T (0.69) C (0.31) |
||
| G/C SNP | 1146 | G (0.69) C (0.31) |
||
| (GA)n (UU18L8) |
−68395 | 139 (0.29) 143 (0.68) 147 (0.03) |
||
| htr2A | C/T SNP | IVS 2-10 | C (0.85) T (0.15) |
128-C (0.07) 130-C (0.41) 132-C (0.37) 132-T (0.15) |
| (CA)n (UUHTR2AEX2) |
IVS2 + 1439 | 128 (0.07) 130 (0.41) 132 (0.52) |
||
| slc6A4 | C/T SNP | 411 | C (0.75) T (0.25) |
C-G (0.75) T-A (0.25) |
| G/A SNP | IVS9-12 | G (0.75) A (0.25) |
ahtr1A, htr1B, htr2A = respectively serotonin receptor 1A, 1B, and 2A gene; slc6A4 = serotonin transporter gene
bSNP = single nucleotide polymorphism. Names of microsatellite markers have been included in brackets
cPosition refers to the coding sequence of the canine gene. We used the nomenclature recommended by den Dunnen and Antonarakis (2001): the A of the ATG start codon is designated number 1, the nucleotide 5′ to this A is numbered −1, and the nucleotide 3′ of the translation termination codon is *1. Positions in introns refer to the nearest exon. The nomenclature of the introns is based on the human gene structure. IVS = intervening sequence
dAllele and haplotype frequencies were determined in a group of 27 (htr1A and htr2A), 31 (htr1B), or 26 (slc6A4) parents
eThis polymorphism is nonsynonymous