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(A) Dose-dependent effects of dexamethasone on calcification of neonatal rat VSMCs. (B) Dose-dependent effects of ascorbic acid on calcification of neonatal rat VSMCs. VSMCs were treated for 21 days with calcification medium containing varying amounts of dexamethasone or ascorbic acid. Calcium deposition was quantified by o-cresolphthalein method. The data are presented as mean ± SEM (n = 6–12). #P < 0.05 vs. all other treatments. ◆P < 0.05 vs. 0 and 5 μg/ml ascorbic acid
